lytA-based identification methods can misidentify Streptococcus pneumoniae
نویسندگان
چکیده
منابع مشابه
Identification of Streptococcus pneumoniae revisited.
The sensitivities and specificities of several different diagnostic assays for Streptococcus pneumoniae were assessed using 99 clinical isolates of S. pneumoniae and 101 viridans streptococci and were as follows: Pneumoslide, 99 and 87%, respectively; Directigen, 100 and 85%, respectively; Phadebact, 100 and 98%, respectively; deoxycholate drop test, 99 and 98%, respectively; deoxycholate tube ...
متن کاملThe Identification of Streptococcus pneumoniae
Streptococcus pneumoniae, pneumococcus, is an important human pathogen that causes both serious invasive infections, such as septicaemia, meningitis and pneumonia, as well as mild upper respiratory infections. It also belongs to the normal nasopharyngeal microbial flora. The purpose of this study was to compare bacteriological phenotypic methods with genetechnological methods in the identificat...
متن کاملDetection of lytA, pspC, and rrgA genes in Streptococcus pneumoniae isolated from healthy children
BACKGROUND AND OBJECTIVES Many surface proteins are implicated in nasopharyngeal colonization and pathogenesis of Streptococcus pneumoniae. Some of these factors are candidate antigens for protein based vaccines. New vaccine designs focus on the surface proteins (e. g., pspA and pspC) and also cytolysin, and pneumolysin. In this study, 3 key virulence genes, lytA, pspC, and rrgA, which encoded ...
متن کاملStructural and Functional Insights into Peptidoglycan Access for the Lytic Amidase LytA of Streptococcus pneumoniae
UNLABELLED The cytosolic N-acetylmuramoyl-l-alanine amidase LytA protein of Streptococcus pneumoniae, which is released by bacterial lysis, associates with the cell wall via its choline-binding motif. During exponential growth, LytA accesses its peptidoglycan substrate to cause lysis only when nascent peptidoglycan synthesis is stalled by nutrient starvation or β-lactam antibiotics. Here we pre...
متن کاملReal-Time Assay as A Tool for Detecting lytA Gene in Streptococcus pneumoniae Isolates
OBJECTIVE In-time diagnosis of Streptococcus pneumoniae (S. pneumonia) can play a significant role in decreasing morbidity and mortality rate. Applying molecular methods has gained popularity due to the existing limits of routine diagnostic methods. Examining the expression of different genes of this bacterium through different molecular methods suggests that lytA gene has a higher sensitivity ...
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ژورنال
عنوان ژورنال: Diagnostic Microbiology and Infectious Disease
سال: 2016
ISSN: 0732-8893
DOI: 10.1016/j.diagmicrobio.2016.03.018